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Image Search Results
Journal: eLife
Article Title: Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function
doi: 10.7554/eLife.84168
Figure Lengend Snippet:
Article Snippet: The Taqman assays (
Techniques: Recombinant, Plasmid Preparation, Sequencing, TaqMan Assay, DNA Extraction, Isolation, Sample Prep, Picogreen Assay
Journal: Acta Neuropathologica
Article Title: Mitochondrial bioenergetic deficits in C9orf72 amyotrophic lateral sclerosis motor neurons cause dysfunctional axonal homeostasis
doi: 10.1007/s00401-020-02252-5
Figure Lengend Snippet: Mitochondrial DNA copy number in human C9orf72 post-mortem spinal cord tissue is unaltered. a Representative photomicrographs showing DNA in situ hybridisation in control ventral horn spinal cord tissue. Red spots denote probe binding to MT-CO1 —a surrogate of mitochondrial DNA copy number. RNase treatment resulted in no change in signal, while DNase treatment resulted in elimination of signal, confirming that the probe binds to DNA, and not RNA. Tissue was counterstained with haematoxylin and images were acquired at 40 × magnification. Scale bars = 20 µm. b Representative photomicrographs showing unaltered mitochondrial copy number in a C9orf72 hexanucleotide repeat expansion mutation case compared with its age- and sex-matched control, both in spinal cord ventral horn motor neurons (upper panel) and dorsal horn sensory neurons (lower panel). Red spots denote probe binding to MT-CO1 . Tissue was counterstained with haematoxylin and images were acquired at 40 × magnification. Scale bars = 20 µm. Bar charts depict the quantification of the number of spots per neuron in either the ventral (upper panel) or dorsal horn (lower panel). Bars represent aggregate mean ± SEM, with each dot representing the mean count for ten cells for each of the five post-mortem specimens for C9orf72 -ALS (red bars) and their age- and sex-matched controls (blue bars). Statistical significance was evaluated with the Mann–Whitney test; ‘ns’ denotes non-significant result ( p ≥ 0.05)
Article Snippet: Probe hybridisation was then performed by incubating the slides with four drops of custom-designed BaseScope™ probe (BA-Hs-MT-CO1-3zz-st-sense; 891111, Advanced Cell Diagnostics™
Techniques: In Situ, Hybridization, Binding Assay, Mutagenesis, MANN-WHITNEY